施用冷杉针叶三萜酸对水稻产量和品质的影响

以镇稻8号为材料,研究了不同用量冷杉针叶三萜酸对水稻产量和品质的影响。结果表明,与喷清水对照相比较,施用冷杉针叶三萜酸后能够提高水稻千粒重和产量,并能提高稻米的糙米率、整精米率、直链淀粉含量和蛋白质含量,改善稻米碾磨品质和营养品质。10%冷杉针叶三萜酸水乳液最适施药方法为:播种前拌种剂量80 mL/t、分蘖期和抽穗期喷施剂量80 mL/hm~2,能使水稻增产16.60%。     

Replacement of Fishmeal by a Mixture of Soybean Meal and Chlorella Meal in Practical Diets for Juvenile Crucian Carp,Carassius auratus

A growth trial was conducted to evaluate the effect of a mixture of soybean meal and Chlorella meal (SCM) as a dietary fishmeal (FM) substitute on growth performance, apparent digestibility coefficients (ADCs), digestive enzymatic activities, and histology of juvenile crucian carp, Carassius auratus. Five isonitrogenous and isoenergetic diets were formulated to replace 0 (SCM0), 25 (SCM25), 50 (SCM50), 75 (SCM75), and 100% (SCM100) of protein from FM with SCM, respectively. The diets were fed to triplicate groups of juvenile crucian carp for 6 wk. Weight gain, specific growth rate, feed intake, protein efficiency ratio, and intestinal digestive enzymatic activities (amylase, trypsin, and lipase) tended to decline with increasing FM replacement levels (P > 0.05). Dietary SCM substitution significantly influenced dry matter content in muscle, and crude protein and lipid contents in liver (P < 0.05). ADCs for dry matter, protein, lipid, energy, and most amino acids showed no significant differences between the control and SCM25 group, but tended to decline with replacement levels over 25%. Higher SCM substitution (50–100%) caused karyopyknosis and necrosis in liver, but intestinal histology did not show noticeable pathological changes. The present study indicated that FM could be replaced by 25% of SCM, without significant adverse growth performance, feed utilization, and histology of crucian carp.     

Growth performance and whole‐body composition of yellow catfish (Pelteobagrus fulvidraco Richardson) under feeding restriction

Fish growth is an important index in aquaculture practice. However, the effect of growth under restricted feeding on quality of fish grown to the same size remains poorly unknown. In this study, yellow catfish were reared by feeding them at three different feeding rates such as low (FR‐L group), medium (FR‐M group) and high (apparent satiation) ration levels for 82, 58 and 34 days to reach the same size, respectively. After this period, the growth performance, whole‐body amino acid composition and fatty acid profile of the fish were evaluated. Feeding restriction led to significant decreases in specific growth rate and feed conversion efficiency. Significant increases in dry matter, protein and energy contents were observed in fish under both restricted feeding treatments. FR‐L‐treated fish had significantly lower whole‐body essential amino acid contents, and significantly higher Ala and non‐essential amino acid contents compared to fish fed to satiation. Fish in restricted feeding treatments had significantly higher n‐3 PUFA and n‐3/n‐6 ratio compared to the fish fed to satiation. The present results indicate that from a quality perspective, there are certain advantages in improvement of whole‐body composition and enrichment of n‐3 PUFA associated with the restricted feeding of yellow catfish at slow growth rate.     

Influence of dietary lipid on growth performance and some lipogenesis‐related gene expression of tongue sole (Cynoglossus semilaevis) larvae

A 30‐day feeding trial was conducted to investigate the effects of dietary lipid levels on growth performance, activities of digestive enzymes, fatty acid composition and some lipogenesis‐related gene expression of half‐smooth tongue sole (Cynoglossus semilaevis) larvae. Five isoproteic diets were formulated with graded lipid levels (6.68%, 9.84%, 13.47%, 17.89% and 21.88% dry weight) using fish oil as the main lipid source. Each diet was randomly allocated to triplicate groups of 150 larval tongue sole (35 DAH, 54 ± 1 mg). Fish were fed five times daily to apparent satiation during the feeding experiment. Results showed that, the survival rate (SR) of larvae increased significantly firstly, and thereafter decreased significantly. The specific growth rates (SGR) of larvae fed the diet with 13.47% lipid were significantly higher than other treatments. Larvae fed 9.84% or 13.47% dietary lipid showed higher trypsin, lipase, leucine aminopeptidase and alkaline phosphatase (AP) activities than other treatments, whereas amylase activity nearly showed reverse trend with them. The fatty acid composition of the tongue sole larvae was well correlated with dietary fatty acid profile. Expression of acetyl‐CoA carboxylase alpha (ACC1) was found to be slightly negatively correlated with dietary lipid level, and high dietary lipid level depressed the expressions of acetyl‐CoA carboxylase beta (ACC2) and fatty acid synthase (FAS) mRNA expression significantly, implying that larvae may cope with high dietary lipid mainly through down‐regulating lipogenesis‐related gene expression of FAS and ACC2. Besides, on the basis of SGR, the optimal dietary lipid level for larval tongue sole was estimated to be 13.56% using second‐order polynomial curve.     

Effect of different feeds on the growth,survival and reproductive performance of zebrafish,Danio rerio (Hamilton, 1822)

Present experiment was conducted to determine the effect of different feeds with varying protein levels on the growth, survival and reproductive performance of zebrafish, Danio rerio. The control diet (T1) was wild‐collected zooplankton from local fish ponds, while test diets with 350 g kg^?1 protein (T2), 400 g kg^?1 protein (T3) and 450 g kg^?1 protein (T4) were formulated and fed to fish for a period of 210 days. The significantly (P < 0.05) highest mean weight gain and specific growth rate were observed in T1, which were similar with T3 and T4. The significantly (P < 0.05) highest number of egg production per female and relative fecundity were found in T1, followed by T4 and T3 while T2 produced lowest number of eggs. No significant (P > 0.05) differences were observed in brood survival rate, fertilization and hatching rate among the dietary treatments. The highest (P < 0.05) fry survival rate was recorded in T1, followed by T3 and T4. Thus, it is suggested that control diet i.e. mixed zooplankton exhibited better growth, reproductive performance and fry survival rate. However, diet containing 400 g kg^?1 crude protein also gave comparable results in terms of growth, survival and reproductive performance of zebrafish.     

Single nucleotide polymorphisms in the growth hormone gene of Oreochromis niloticus and their association with growth performance

Polymorphisms in the growth hormone (GH) gene that is associated with the growth rate of farmed fish have been the target of many breeding programmes. The present study aimed to identify single nucleotide polymorphisms (SNPs) in GH gene regions to evaluate the association of SNP variations with the growth rate of two Nile tilapia: Oreochromis niloticus (Linnaeus, 1758) strains. The targeted regions were amplified, sequenced, aligned and screened for the presence of SNPs; thereafter, performance tests were used to check for the association between SNPs and weight. Allele and genotype frequencies were estimated for each SNP and genotype. Genotype blocks or sets of SNP genotypes and frequencies were also estimated. Association between SNPs and growth rate was statistically evaluated using a univariate linear mixed model that included both fixed and random effects. A total of 10 SNPs were identified, nine in the proximal promoter and one located in the 5′ UTR, forming 10 genotype blocks. In all weight recordings, five genotype blocks were significantly associated with the highest weights. Single nucleotide polymorphisms 6‐10 were also found to be significantly associated with growth (p‐value < .05). Genotypes with higher additive genetic values for weight were identified in the Chitralada strain, suggesting a possible impact of these additive effects of the GH SNP genotype on the growth rate of Nile tilapia. These findings may be used as part of marker‐assisted selection in tilapia breeding programmes.     

Significant improvement of shrimp growth performance by growth hormone‐releasing peptide‐6 immersion treatments

Growth hormone‐releasing peptide‐6 (GHRP‐6) is one of the earliest developed synthetic peptidyl growth hormone secretagogue receptor agonists. These compounds mimic the effect of the endogenous ligand ghrelin. In vertebrates, ghrelin is a potent circulating orexigenic hormone with functional roles in controlling food intake, energy expenditure, adiposity, growth hormone secretion and immunity. Ghrelin has been studied mainly in vertebrates; thus, little is known about its role in invertebrates, including crustaceans. We first evaluated the effect of GHRP‐6 injection over feed intake in shrimp and its effects on shrimp growth when the peptide was administrated by successive immersion baths. GHRP‐6 increased feed intake, body weight and size, the number of rostral spines and gill branches, protein concentration and haemocyte number in treated shrimps. We also evaluated the peptide uptake and clearance in a pharmacokinetics, using [H³]GHRP‐6 administered to postlarvae. Given a limited exposure and efficient clearance of the peptide‐associated radioactivity from larvae, our findings suggested that GHRP‐6‐treated Litopenaeus vannamei can be consumed safely by humans after aquaculture applications. These results propose that GHRP‐6 could be an additional tool to study growth physiology in crustaceans and also a promising candidate for development into a new biotechnology product for improving shrimp growth and quality.     

8个格木天然林种源苗期生长差异与早期选择

为研究格木不同天然种源家系苗的生长差异,选出相对速生的优良种源,本研究对8个格木天然林种源、110个家系1年生的苗高和地径等生长量指标进行测量,结合各家系的种子表型性状进行方差分析、多重比较、相关性分析和聚类分析。结果表明:不同种源的格木苗期生长量差异显著,具备种源选择的基础;不同种源的苗期生长量差异主要来源于种源内,具备从优良种源内选择优良家系的基础;苗期生长量与种子大小、种子厚和千粒重等性状呈极显著正相关关系,大粒、饱满种子可作为优良种源和家系选择的初选参考指标。初步选出浦北、北流种源作为格木优良种源。本研究为格木速生种源及家系的早期选择提供依据。     

油菜生长过程对核桃凋落叶化感作用的响应

为研究农作物白菜型油菜生长过程对核桃凋落叶化感作用的生理响应，采用盆栽试验，通过设置空白对照CK（不添加凋落叶）和3个凋落叶添加剂量水平:L₆₀（60 g·盆^-1）、L₁₂₀（120 g·盆^-1）和L₁₈₀（180 g·盆^-1），测定盆栽油菜生长中后期的营养生长、生殖生长及生理响应相关指标。结果表明:1）核桃凋落叶分解对油菜中期营养生长（地径、株高）有显著抑制，在后期得到明显减弱，可仍对后期油菜生殖生长（开花性状、结实性状和产量）产生抑制影响；但这种抑制效应不会影响油菜结实种子的萌发。2）油菜生长中期（100 d），随核桃凋落叶剂量增大，油菜叶片中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性逐步增强，过氧化物酶（POD）活性无显著变化；丙二醛（MDA）含量仅在L₁₈₀处理下显著>CK（P＜0.05），可溶性糖（SS）含量逐步增加，而可溶性蛋白（SP）含量呈逐步下降；到油菜生长后期（140 d）时，各处理下油菜POD、CAT的活性和SS、SP的含量均逐步下降，MDA含量均显著P<0.05）。3）各核桃凋落叶处理对油菜叶片各类叶绿素均有明显抑制作用,随凋落叶添加量增加，油菜叶片净光合速率（P_n）、气孔导度（G_s）和胞间CO₂浓度（C_i）呈先降后增的趋势。综上所述，核桃凋落叶经土壤分解对油菜中后期生长造成强烈的化感胁迫，其主要通过对油菜的细胞膜造成过氧化伤害，使叶绿素合成受到抑制，光合作用受到影响，进而妨碍其生长及产量品质的形成。